Get Induction of targeted heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease
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The RNA-guided Cas9 system represents a flexible approach for genome editing in plants. This method can create specific mutations that knock-out or alter target gene function. It provides a valuable tool for plant research and offers opportunities for crop improvement. We demonstrate the use of RNA-guided Cas9 to generate mutations in target genes of both barley and B. oleracea and show stable transmission of these mutations thus establishing the potential for rapid characterisation of gene function in these species. In addition, the off-target effects reported offer both potential difficulties and specific opportunities to target members of multigene families in crops. Proceeds from the sale of this book go to the support of an elderly disabled person. BioMed Central The high level of genetic diversity between individual tumors suggests that if it were to be developed a broad cancer vaccine would be unlikely to work for more than Efficient and transgene-free - Nature Communications The CRISPR (clustered regularly interspaced short palindromic repeats)/associated nuclease Cas9 system has been widely used in plants to introduce targeted mutations
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